Title
PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid
SKU
RXSOL-60-6605-260
Supply Location::
Locations
[PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid] manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman.
Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada.
[PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid] is available in small packing as well as in bulk. Buy premium quality [PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid] and other lab chemicals from one of the most trusted brands.
Category
Lab Chemical-60
SHORT DESCRIPTION:
PRODUCT REMARK:
TAG IDENFIFICATION:
FIELD NOTE:
PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic acid)) PIPES is a biological buffer (zwitterionic) designed by Good, et al. and typically referred to as Good's buffer useful in cell culture media formulations.
Mild detergent extraction of chick brain synaptic plasma membranes followed by gel electrophoresis suggests that synaptic plasma membrane tubulin is an integral component. Although some of the synaptic plasma membrane tubulin might be aggregates, that possibility is not supported by the observation that tubulin aggregates that are added to synaptosomes before synaptic subfractionation do not partition with synaptic plasma membranes during membrane isolation.
PIPES has two pKa values. One pKa (6.76 at 25°C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25° C. The second pKa value is at 2.67 with a buffer range of from 1.5 - 3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.